SCIENTIFIC WORKPACKAGES DESCRIPTION:
WORKPACKAGE 1 & 2 – Management and scientific Coordination
WORKPACKAGE 3 – Imaging process morphing patient specific implant shape and optimized internal macro-structure
- Mathematical modeling for the choice of the most suitable implant scaffold in relation to bone defect size and location;
- Analyze and process retrospective clinical CT cases affected by unilateral orbital floor fractures to provide computer templates matching the shape and size of the orbital defects;
- Development of model scaffold architectures for improved cells seeding efficiency and mass transport;
- Morphing of clinical image data and design internal architectures for rapid prototyping technologies.
WORKPACKAGE 4 – Rapid Prototyping technologies and advance biomaterials incorporating optimized macro-architecture
- Prepare and characterize calcium phosphate particles;
- Prepare and characterize PTMC resin, PTMC/CaP composite, PLGA microspheres/nanofibers loaded with Icaritin and their combination thereof;
- Develop and optimize SLA technology for preparation of custom-made scaffolds with controlled and precise macro-architecture;
- Develop and characterize SLA scaffolds releasing biological effectors (e.g. Icaritin).
WORKPACKAGE 5 – In vitro bone tissue engineering
To define optimal scaffold properties in combination or not with hydrogel carrier for the seeding and differentiation of human mesenchymal stromal cells (MSCs) into bone in vitro. Within this WP, the influence of the following parameters will be examine:
(1) PTMC/CaP ratio,
(2) scaffold architecture,
(3) quantity and released Icaritin from loaded microspheres.
WORKPACKAGE 6 – In vivo bone tissue engineering
In this WP, depending on the outcome of WPs 2- 4, small and large animal models are proposed to assess orbital floor fracture. Ideally, if the WPs 1-3 will be successful in developing a blue print of custom-made scaffold for bone tissue engineering and identifying in vitro, the best conditions to repair bone, small animal model will be performed (rabbit calvarial defect). Alternatively, if WPs 2-4 did not reach all their target, specific models addressing biocompatibility, pharmacokinetics, etc. will be proposed. A pilot study will be performed on a large animal model using the best custom-made biomaterial based on the finding of the EU and Chinese partners.
WORKPACKAGE 7 – Consolidate EU-China biomaterials research and further enhance collaboration
- Disseminate information on the research performed to the consortium, the scientific community, the AO orthopedic surgeons network, the industry throughout Europe, and China;
- Ensure proper exploitation of RAPIDOS Project.